Journal article
Quantitative phospho-proteomics reveals the Plasmodium merozoite triggers pre-invasion host kinase modification of the red cell cytoskeleton
ES Zuccala, TJ Satchwell, F Angrisano, YH Tan, MC Wilson, KJ Heesom, J Baum
Scientific Reports | Published : 2016
DOI: 10.1038/srep19766
Abstract
The invasive blood-stage malaria parasite-the merozoite-induces rapid morphological changes to the target erythrocyte during entry. However, evidence for active molecular changes in the host cell that accompany merozoite invasion is lacking. Here, we use invasion inhibition assays, erythrocyte resealing and high-definition imaging to explore red cell responses during invasion. We show that although merozoite entry does not involve erythrocyte actin reorganisation, it does require ATP to complete the process. Towards dissecting the ATP requirement, we present an in depth quantitative phospho-proteomic analysis of the erythrocyte during each stage of invasion. Specifically, we demonstrate exte..
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Awarded by Australian Research Council
Funding Acknowledgements
We thank Ashley Toye for substantial contributions towards preliminary invasion proteomics and for helpful discussions and support. We thank the Toye laboratory for hosting ESZ as part of an Australian Society for Parasitology Network Researcher Exchange Award and Melbourne University Overseas Research Experience and John & Betty McCreery Traveling Scholarships to undertake proteomics work at the University of Bristol. We also thank Danny Wilson, Danushka Marapana and Maya Olshina for experimental support during this project. Experimental data concerning parasite cell and molecular biology presented here was made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. The research was directly supported by a National Health and Medical Research Council of Australia (NHMRC) Project Grant (APP1047085 JB), a project grant from the Wellcome Trust (094277, TJS) and the National Health Service (NHS) Blood and Transplant Research and Development programme. ESZ was supported by an Australian Postgraduate Award and FA was supported through a NHMRC Dora Lush Scholarship (APP1055246). JB was supported through a Future Fellowship (FT100100112) from the Australian Research Council (ARC) and is currently supported by the Wellcome Trust through a New Investigator Award (100993/Z/13/Z).